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wnt β catenin pathway inhibitor dickkopf related protein 1  (MedChemExpress)


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    MedChemExpress wnt β catenin pathway inhibitor dickkopf related protein 1
    Wnt β Catenin Pathway Inhibitor Dickkopf Related Protein 1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wnt+%CE%B2+catenin+pathway/DKK-1%2C+Mouse/pm42860190-133-9-18
    Average 92 stars, based on 4 article reviews
    wnt β catenin pathway inhibitor dickkopf related protein 1 - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Injection:

    Article Title: Inhibition of Colon Cancer Xenograft Growth by Celecoxib Analog OSU-03013 Through Wnt/β-Catenin Pathway Modulation
    Article Snippet: .. To investigate the effects of inhibiting the Wnt/ β-catenin pathway on tumor growth and angiogenesis, mice in the OSU-03013 group were injected intraperitoneally with 1 mL of the Wnt/β-catenin pathway inhibitor MSAB (MedChemExpress), the Wnt/β-catenin pathway agonist TWS119 (Selleck, Houston, TX, USA), or the vehicle 1% DMSO at a dose of 20 mg/kg. ..

    Concentration Assay:

    Article Title: ATF5 inhibits autophagy and the Wnt/β-catenin pathway by upregulating mTOR to suppress the stemness of liver cancer stem cells.
    Article Snippet: Background: Hepatocellular carcinoma (HCC) is the third most common cause of cancer-related death worldwide, and cancer stem cells (CSCs) are considered risk factors for HCC progression.. Research has indicated a link between reduced ATF5 expression and the aggressive growth of HCC, yet the effect of ATF5 on the stemness of HCC is still ambiguous.. Therefore, the aim of this study is to explore the role and potential mechanism of ATF5 in the stemness of HCC.

    Article Title: Effects of SOX10 on the proliferative, invasive, migratory, and epithelial–mesenchymal transition abilities of triple-negative breast cancer cells
    Article Snippet: .. The Wnt/β-catenin pathway was activated using CHIR-99021 (a selective GSK-3β inhibitor; MedChemExpress, Monmouth Junction, New Jersey, United States, Cat. No. HY-10182) at a concentration of 5 μM , and inhibited using XAV939 (a tankyrase 1/2 inhibitor; MedChemExpress, Cat. No. HY-15147) at a concentration of 10 μM ( ). ..

    Blocking Assay:

    Article Title: ATF5 inhibits autophagy and the Wnt/β-catenin pathway by upregulating mTOR to suppress the stemness of liver cancer stem cells.
    Article Snippet: Background: Hepatocellular carcinoma (HCC) is the third most common cause of cancer-related death worldwide, and cancer stem cells (CSCs) are considered risk factors for HCC progression.. Research has indicated a link between reduced ATF5 expression and the aggressive growth of HCC, yet the effect of ATF5 on the stemness of HCC is still ambiguous.. Therefore, the aim of this study is to explore the role and potential mechanism of ATF5 in the stemness of HCC.

    Article Title: Meis1 Negatively Regulates Epithelial-Mesenchymal Transition via Wnt/β-catenin Pathway in Oral Submucous Fibrosis
    Article Snippet: TGF-β1 (Novoprotein, CA59) induced HaCaT cells to construct a cell model of OSF. .. MSAB was used to treat HaCaT cells by blocking the Wnt/β-catenin pathway (MCE, HY-120697). ..

    Saline:

    Article Title: Electroacupuncture ameliorates cognitive dysfunction in T2DM rats by modulating astrocytic polarization and aberrant energy metabolism in the hippocampus via the Wnt/β-catenin pathway
    Article Snippet: .. In the EA + DKK1 group, Dickkopf-1 (DKK1), a specific inhibitor of the Wnt/β-catenin pathway, was administered intranasally at a dose of 2 mg/kg (prepared in saline; MCE, China) once daily for nine consecutive weeks. ..

    other:

    Article Title: Proanthocyanidins Ameliorate LPS-Inhibited Osteogenesis of PDLSCs by Restoring Lysine Lactylation
    Article Snippet: Grape seed oligomeric proanthocyanidins were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Transfection:

    Article Title: SP1‑mediated ADAMTS5 transcription promotes IL‑1β‑induced chondrocyte injury via Wnt/β‑catenin pathway in osteoarthritis
    Article Snippet: CHON-001 cells were stimulated with different concentrations (0, 5, 10 and 15 ng/ml) of IL-1β at 37°C for 24 h to establish the OA cell model. For transfection, cells were transfected with 50 nM ADAMTS5 siRNA [si-ADAMTS5, forward (F) 5′-AAAAUGUUUGGAUUCGUGCUC-3′; reverse (R) 5′-GCACGAAUCCAAACAUUUUCC-3′], 50 nM SP1 siRNA (si-SP1, F 5′-ACUUGAUACUGAAUAUUAGGC-3′; R 5′-CUAAUAUUCAGUAUCAAGUAA-3′), 4.0 μg pcDNA3.1 ADAMTS5 overexpression vector (F 5′-AAAGGGGAGAATCTGCCTGC-3′; R 5′-CCAAGATCCCCAGTTGCCAT-3′), 4.0 μg pcDNA3.1 SP1 overexpression vector (F 5′-GTCCGCCCTCTGACCAAG-3′; R 5′-AAGGCACCACCACCATTACC-3′) or negative controls (si-NC, F 5′-GGAGUAGGGAGCAAACCUAUAGGAA-3′, R 5′-UUCCUAUAGGUUUGCUCCCUACUCC-3′; pcDNA3.1, F 5′-CTAGAGAACCCACTGCTTAC-3′, R 5′-TAGAAGGCACAGTCGAGG-3′) using Lipofectamine ® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) at 37°C for 24 h. All siRNAs and pcDNAs were purchased from Guangzhou RiboBio Co., Ltd. After 24 h post-transfection, cells were treated with 10 ng/ml IL-1β for 24 h at 37°C. .. To explore the role of Wnt/β-catenin pathway in the regulation of ADAMTS5 on IL-1β-induced CHON-001 cell injury, CHON-001 cells were transfected with 50 nM si-NC/si-ADAMTS5 for 24 h and treated with 10 ng/ml IL-1β and 40 μM Wnt/β-catenin pathway agonist SKL2001 (MedChemExpress, Inc.) for 24 h at 37°C. ..



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    PPOX knockdown inhibits the migratory and invasive capacities of ccRCC cells <t>and</t> <t>downregulates</t> <t>Wnt/β-catenin</t> signaling pathway activity. (A) Wound healing assay confirmed that PPOX knockdown inhibited cell migration (magnification, 40×). (B) Transwell assay confirmed that PPOX knockdown inhibited cell invasion (stained with crystal violet; magnification, 100×). (C) PPOX knockdown reduced Wnt/β-catenin pathway activity. All experiments were performed with at least three independent replicates. Statistical comparisons between two groups were analyzed by unpaired Student’s t-test using GraphPad Prism 8. Scale bar: 100 μm. NC, negative control; PPOX, protoporphyrinogen oxidase.
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    PPOX knockdown inhibits the migratory and invasive capacities of ccRCC cells and downregulates Wnt/β-catenin signaling pathway activity. (A) Wound healing assay confirmed that PPOX knockdown inhibited cell migration (magnification, 40×). (B) Transwell assay confirmed that PPOX knockdown inhibited cell invasion (stained with crystal violet; magnification, 100×). (C) PPOX knockdown reduced Wnt/β-catenin pathway activity. All experiments were performed with at least three independent replicates. Statistical comparisons between two groups were analyzed by unpaired Student’s t-test using GraphPad Prism 8. Scale bar: 100 μm. NC, negative control; PPOX, protoporphyrinogen oxidase.

    Journal: Translational Andrology and Urology

    Article Title: Comprehensive bioinformatics and experimental analysis of PPOX reveals its carcinogenic effect in clear cell renal cell carcinoma

    doi: 10.21037/tau-2026-1-0024

    Figure Lengend Snippet: PPOX knockdown inhibits the migratory and invasive capacities of ccRCC cells and downregulates Wnt/β-catenin signaling pathway activity. (A) Wound healing assay confirmed that PPOX knockdown inhibited cell migration (magnification, 40×). (B) Transwell assay confirmed that PPOX knockdown inhibited cell invasion (stained with crystal violet; magnification, 100×). (C) PPOX knockdown reduced Wnt/β-catenin pathway activity. All experiments were performed with at least three independent replicates. Statistical comparisons between two groups were analyzed by unpaired Student’s t-test using GraphPad Prism 8. Scale bar: 100 μm. NC, negative control; PPOX, protoporphyrinogen oxidase.

    Article Snippet: The canonical Wnt/β-catenin pathway was activated using CHIR-99021 (MedChemExpress, Cat# HY-10182), a selective glycogen synthase kinase-3β (GSK-3β) inhibitor.

    Techniques: Knockdown, Activity Assay, Wound Healing Assay, Migration, Transwell Assay, Staining, Negative Control

    PPOX overexpression enhances the proliferative, migratory, and invasive capacities of ccRCC cells and upregulates Wnt/β-catenin pathway activity. (A) PPOX overexpression efficiency in A498 and 769-P cells. (B-D) CCK-8, colony formation, and EdU assays (EdU and DAPI staining; magnification, 100×) confirmed that cell proliferation was elevated after PPOX overexpression. (E,F) Wound healing (40×) and Transwell assays (stained with crystal violet; magnification, 100×) confirmed that PPOX overexpression enhances cell migration and invasion. (G) PPOX overexpression enhanced Wnt/β-catenin pathway activity. All experiments were performed with at least three independent replicates. Statistical comparisons between two groups were analyzed by unpaired Student’s t-test using GraphPad Prism 8. Scale bar: 100 μm. NC, negative control; OD, optical density; OE, overexpression; PPOX, protoporphyrinogen oxidase.

    Journal: Translational Andrology and Urology

    Article Title: Comprehensive bioinformatics and experimental analysis of PPOX reveals its carcinogenic effect in clear cell renal cell carcinoma

    doi: 10.21037/tau-2026-1-0024

    Figure Lengend Snippet: PPOX overexpression enhances the proliferative, migratory, and invasive capacities of ccRCC cells and upregulates Wnt/β-catenin pathway activity. (A) PPOX overexpression efficiency in A498 and 769-P cells. (B-D) CCK-8, colony formation, and EdU assays (EdU and DAPI staining; magnification, 100×) confirmed that cell proliferation was elevated after PPOX overexpression. (E,F) Wound healing (40×) and Transwell assays (stained with crystal violet; magnification, 100×) confirmed that PPOX overexpression enhances cell migration and invasion. (G) PPOX overexpression enhanced Wnt/β-catenin pathway activity. All experiments were performed with at least three independent replicates. Statistical comparisons between two groups were analyzed by unpaired Student’s t-test using GraphPad Prism 8. Scale bar: 100 μm. NC, negative control; OD, optical density; OE, overexpression; PPOX, protoporphyrinogen oxidase.

    Article Snippet: The canonical Wnt/β-catenin pathway was activated using CHIR-99021 (MedChemExpress, Cat# HY-10182), a selective glycogen synthase kinase-3β (GSK-3β) inhibitor.

    Techniques: Over Expression, Activity Assay, CCK-8 Assay, Staining, Migration, Negative Control

    PPOX regulates malignant progression of ccRCC through the Wnt/β-catenin pathway. (A) TOP/FOP Flash reporter activity ratios in A498 and 769-P cells following PPOX knockdown or overexpression. (B) Protein expression levels of β-catenin, c-Myc and CCND1 in A498 and 769-P cells after PPOX knockdown and CHIR-99021 treatment. (C) Cell proliferation assessed by EdU assays (magnification, 100×) under PPOX knockdown and CHIR-99021 treatment. (D) Cell migration evaluated by wound healing assays (magnification, 40×). (E) Cell invasion analyzed by Transwell assays (stained with crystal violet; magnification, 100×). All experiments were performed with at least three independent replicates. Two-group comparisons were analyzed using the unpaired t-test, whilst multiple groups (>2) were compared using one-way ANOVA, followed by Tukey’s HSD test for post hoc pairwise comparisons where applicable. Scale bar: 100 μm. ANOVA, analysis of variance; ccRCC, clear cell renal cell carcinoma; HSD, Honestly Significant Difference; NC, negative control; PPOX, protoporphyrinogen oxidase.

    Journal: Translational Andrology and Urology

    Article Title: Comprehensive bioinformatics and experimental analysis of PPOX reveals its carcinogenic effect in clear cell renal cell carcinoma

    doi: 10.21037/tau-2026-1-0024

    Figure Lengend Snippet: PPOX regulates malignant progression of ccRCC through the Wnt/β-catenin pathway. (A) TOP/FOP Flash reporter activity ratios in A498 and 769-P cells following PPOX knockdown or overexpression. (B) Protein expression levels of β-catenin, c-Myc and CCND1 in A498 and 769-P cells after PPOX knockdown and CHIR-99021 treatment. (C) Cell proliferation assessed by EdU assays (magnification, 100×) under PPOX knockdown and CHIR-99021 treatment. (D) Cell migration evaluated by wound healing assays (magnification, 40×). (E) Cell invasion analyzed by Transwell assays (stained with crystal violet; magnification, 100×). All experiments were performed with at least three independent replicates. Two-group comparisons were analyzed using the unpaired t-test, whilst multiple groups (>2) were compared using one-way ANOVA, followed by Tukey’s HSD test for post hoc pairwise comparisons where applicable. Scale bar: 100 μm. ANOVA, analysis of variance; ccRCC, clear cell renal cell carcinoma; HSD, Honestly Significant Difference; NC, negative control; PPOX, protoporphyrinogen oxidase.

    Article Snippet: The canonical Wnt/β-catenin pathway was activated using CHIR-99021 (MedChemExpress, Cat# HY-10182), a selective glycogen synthase kinase-3β (GSK-3β) inhibitor.

    Techniques: Activity Assay, Knockdown, Over Expression, Expressing, Migration, Staining, Negative Control

    Effect of SOX10 knockdown and overexpression on classical Wnt/β-catenin pathway-related protein expression in TNBC cell lines. Protein expression of C-myc, CyclinD1, WNT1, phospho-GSK-3β (Ser9), and nuclear β-catenin was detected by Western blot analysis, with band gray values normalized to GAPDH (total protein) or Lamin B1 (nuclear protein, for β-catenin). (A) Protein expression and quantification in MDA-MB-453 cells. (B) Protein expression and quantification in HCC1937 cells. All experiments were independently repeated three times (n = 3 independent biological replicates), and representative results are shown. Statistical analysis was performed using unpaired two-tailed Student’s t-test for two-group comparisons. siSOX10 vs. siNC, ** P < 0.01; SOX10-OEvs. NC, ## P < 0.01. && indicates P<0.01; $$ indicates P<0.01 for statistical comparisons.

    Journal: Frontiers in Oncology

    Article Title: Effects of SOX10 on the proliferative, invasive, migratory, and epithelial–mesenchymal transition abilities of triple-negative breast cancer cells

    doi: 10.3389/fonc.2026.1769833

    Figure Lengend Snippet: Effect of SOX10 knockdown and overexpression on classical Wnt/β-catenin pathway-related protein expression in TNBC cell lines. Protein expression of C-myc, CyclinD1, WNT1, phospho-GSK-3β (Ser9), and nuclear β-catenin was detected by Western blot analysis, with band gray values normalized to GAPDH (total protein) or Lamin B1 (nuclear protein, for β-catenin). (A) Protein expression and quantification in MDA-MB-453 cells. (B) Protein expression and quantification in HCC1937 cells. All experiments were independently repeated three times (n = 3 independent biological replicates), and representative results are shown. Statistical analysis was performed using unpaired two-tailed Student’s t-test for two-group comparisons. siSOX10 vs. siNC, ** P < 0.01; SOX10-OEvs. NC, ## P < 0.01. && indicates P<0.01; $$ indicates P<0.01 for statistical comparisons.

    Article Snippet: The Wnt/β-catenin pathway was activated using CHIR-99021 (a selective GSK-3β inhibitor; MedChemExpress, Monmouth Junction, New Jersey, United States, Cat. No. HY-10182) at a concentration of 5 μM , and inhibited using XAV939 (a tankyrase 1/2 inhibitor; MedChemExpress, Cat. No. HY-15147) at a concentration of 10 μM ( ).

    Techniques: Knockdown, Over Expression, Expressing, Western Blot, Two Tailed Test

    SOX10 promotes EMT in MDA-MB-453 cells via activating the classical Wnt/β-catenin signaling pathway. (A) Relative protein expression of Wnt/β-catenin pathway-related factors (WNT1, β-catenin, C-myc, and CyclinD1) in siSOX10 MDA-MB-453 cells treated with a Wnt activator, and SOX10-overexpressing MDA-MB-453 cells treated with a Wnt inhibitor. (B) Relative protein expression of EMT-related markers (E-cadherin, N-cadherin, and Vimentin) in the aforementioned treatment groups. All protein levels were detected by Western blot analysis, with band gray values normalized to GAPDH. All experiments were independently repeated three times (n = 3 independent biological replicates), and representative results are shown. Statistical analysis was performed using one-way ANOVA with Tukey’s post-hoc test for multiple comparisons among groups. Compared with MDA-MB-453+siNC, * P < 0.05, ** P < 0.01; compared with MDA-MB-453+siSOX10, # P < 0.05, ## P < 0.01; compared with MDA-MB-453+NC.

    Journal: Frontiers in Oncology

    Article Title: Effects of SOX10 on the proliferative, invasive, migratory, and epithelial–mesenchymal transition abilities of triple-negative breast cancer cells

    doi: 10.3389/fonc.2026.1769833

    Figure Lengend Snippet: SOX10 promotes EMT in MDA-MB-453 cells via activating the classical Wnt/β-catenin signaling pathway. (A) Relative protein expression of Wnt/β-catenin pathway-related factors (WNT1, β-catenin, C-myc, and CyclinD1) in siSOX10 MDA-MB-453 cells treated with a Wnt activator, and SOX10-overexpressing MDA-MB-453 cells treated with a Wnt inhibitor. (B) Relative protein expression of EMT-related markers (E-cadherin, N-cadherin, and Vimentin) in the aforementioned treatment groups. All protein levels were detected by Western blot analysis, with band gray values normalized to GAPDH. All experiments were independently repeated three times (n = 3 independent biological replicates), and representative results are shown. Statistical analysis was performed using one-way ANOVA with Tukey’s post-hoc test for multiple comparisons among groups. Compared with MDA-MB-453+siNC, * P < 0.05, ** P < 0.01; compared with MDA-MB-453+siSOX10, # P < 0.05, ## P < 0.01; compared with MDA-MB-453+NC.

    Article Snippet: The Wnt/β-catenin pathway was activated using CHIR-99021 (a selective GSK-3β inhibitor; MedChemExpress, Monmouth Junction, New Jersey, United States, Cat. No. HY-10182) at a concentration of 5 μM , and inhibited using XAV939 (a tankyrase 1/2 inhibitor; MedChemExpress, Cat. No. HY-15147) at a concentration of 10 μM ( ).

    Techniques: Expressing, Western Blot

    SOX10 promotes EMT in HCC1937 cells via activating the classical Wnt/β-catenin signaling pathway. (A) Relative protein expression of Wnt/β-catenin pathway-related factors (WNT1, β-catenin, C-myc, and CyclinD1) in siSOX10 HCC1937 cells treated with a Wnt activator, and SOX10-overexpressing HCC1937 cells treated with a Wnt inhibitor. (B) Relative protein expression of EMT-related markers (E-cadherin, N-cadherin, and Vimentin) in the aforementioned treatment groups. All protein levels were detected by Western blot analysis, with band gray values normalized to GAPDH. All experiments were independently repeated three times (n = 3 independent biological replicates), and representative results are shown. Statistical analysis was performed using one-way ANOVA with Tukey’s post-hoc test for multiple comparisons among groups. Compared with HCC1937+siNC, ** P < 0.01; compared with HCC1937+siSOX10, ## P < 0.01; compared with HCC1937+NC.

    Journal: Frontiers in Oncology

    Article Title: Effects of SOX10 on the proliferative, invasive, migratory, and epithelial–mesenchymal transition abilities of triple-negative breast cancer cells

    doi: 10.3389/fonc.2026.1769833

    Figure Lengend Snippet: SOX10 promotes EMT in HCC1937 cells via activating the classical Wnt/β-catenin signaling pathway. (A) Relative protein expression of Wnt/β-catenin pathway-related factors (WNT1, β-catenin, C-myc, and CyclinD1) in siSOX10 HCC1937 cells treated with a Wnt activator, and SOX10-overexpressing HCC1937 cells treated with a Wnt inhibitor. (B) Relative protein expression of EMT-related markers (E-cadherin, N-cadherin, and Vimentin) in the aforementioned treatment groups. All protein levels were detected by Western blot analysis, with band gray values normalized to GAPDH. All experiments were independently repeated three times (n = 3 independent biological replicates), and representative results are shown. Statistical analysis was performed using one-way ANOVA with Tukey’s post-hoc test for multiple comparisons among groups. Compared with HCC1937+siNC, ** P < 0.01; compared with HCC1937+siSOX10, ## P < 0.01; compared with HCC1937+NC.

    Article Snippet: The Wnt/β-catenin pathway was activated using CHIR-99021 (a selective GSK-3β inhibitor; MedChemExpress, Monmouth Junction, New Jersey, United States, Cat. No. HY-10182) at a concentration of 5 μM , and inhibited using XAV939 (a tankyrase 1/2 inhibitor; MedChemExpress, Cat. No. HY-15147) at a concentration of 10 μM ( ).

    Techniques: Expressing, Western Blot